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Image Search Results
Journal: Scientific Reports
Article Title: The Regenerative Potential of Amniotic Fluid Stem Cell Extracellular Vesicles: Lessons Learned by Comparing Different Isolation Techniques
doi: 10.1038/s41598-018-38320-w
Figure Lengend Snippet: Comparison of AFSC-EV protein content and EV markers. ( a ) Protein quantification of AFSC-EV preparations using the Pierce Bradford assay. Data are shown as mean ± SD n = 3. No difference was found between preparations isolated with UC, ExoQuick and TEIR. AFSC-EV preparations isolated using qEV had lower protein content than those isolated using Exo-PREP (p < 0.05). ( b ) Correlation analysis between total number of particles analyzed with Nanoparticle Tracking Analysis (Fig. ) and EV protein concentration (μg/μL) in each preparation of AFSC-EVs obtained by different isolation techniques [p = 0.25, r = 0.6 (95% CI −0.56 to 0.97)]. ( c ) Expression of canonical EV markers Hsp70, CD63, Flotillin-1, and TSG101 obtained by Western blot analysis for the different isolation techniques. All AFSC-EV isolation techniques showed no evidence of residual cellular debris, as evidenced by a lack of H3K27me3 protein expression. AFSCs (parent cells) and AFSC-conditioned medium (AFSC-CM; the initial starting material from which all techniques were derived), are shown as positive controls. Representative photo from n = 3 replicate analyses.
Article Snippet: Hsp70, CD63, Flotillin-1, and TSG101 were measured from AFSC-EVs, and from 20 μg total protein from AFSC-CM, and AFSCs using
Techniques: Comparison, Bradford Assay, Isolation, Protein Concentration, Expressing, Western Blot, Derivative Assay